Introduction to Application of Laboratory Equipments

Biosafety and principles of work in laboratory, application of common equipment including micropipette, sampler, vortex, hot plate, water bath, oven, microwave, desiccator, scale, pH meter, centrifuge, hood, incubator, autoclave, a short about solutions preparation (different buffers, physiological serum, PBS, molar, normal solutions, reagents and other current solutions), sample storage method, sample transfer and freezing, safety considerations for working with chemicals (chloroform , guanidine thiocyanate, acrylamide mercaptoethanol, ethidium bromide, phenol, chemical package labels), safety considerations of working with liquid nitrogen, dry ice, ultraviolet light, radioactive materials, in vivo and in vitro methods for determining digestibility and degradability of feeds in animal and poultry such as gas production system, Tilley and Terry, Continuous Culture and in situ method, an abstract of the use of devices such as PCR (extraction DNA, PCR, agarose and polyacrylamide gel, gel staining), Real Time-PCR, ELISA, spectrophotometer, gamma counter, GC, HPLC, electrophoresis, autoanalyzer, atomic absorption spectrometer, amino acid analyzer, freeze dryer, spray dryer, cell counter, coagulometer Electrolyte analyzer, flame -photometer, turbidity meter, viscometer, polarimeter, polarograph, FTIR, TLC, refractometer, fermenter, milkoscan, somatic cell counter, melting meter, Soxhlet, Kjeldahl, Fibrtech, bomb calorimeter, how to prepare histological sections from tissues (working with histokinet device), animal cell and tissue culture.

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